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README.md

Bat Shotgun Metagenomic Flow-work

This is the repository for supplementary materials for the upcoming publication

Differential analysis 馃搳

Files:

  • 馃搼 02_Lab_journal.ipynb - laboratory journal with commands to reproduce pipeline
  • 馃搧 scripts - folder with miscellaneous scripts used for data analysis:
  • 馃搼 Volcano_plots_journal.R - laboratory journal to plot MaAsLin2 results as volcano-plots
  • 馃搼 RA_n_Diversity_journal.R - laboratory journal to plot bar-plots with relative microbial abundance; 伪-diversity metrics as box-plots and 尾-diversity metrics as PCoA plots
  • 馃搼 diff_an.yaml - conda environment

Instruction:

  • Create new environment diff_an
conda env create -f diff_an.yaml

System

This part of work was done using:

  • OS: Ubuntu 22.04 (WSL2 on Windows 11 22H2)
  • RAM: 32GB (16 for WSL2)
  • CPU: Intel Xeon E5-2670v3